Publications by MIVAC researchers 2009

  1. Ahmed, T., A. Lundgren, M. Arifuzzaman, F. Qadri, S. Teneberg, and A. M. Svennerholm. 2009. Children with the Le (a+b-) blood group have increased susceptibility to diarrhea caused by enterotoxigenic Escherichia coli expressing colonization factor I group fimbriae. Infect Immun 77:2059-2064.
  2. Ahmed, T., M. Arifuzzaman, M. Lebens, F. Qadri, and A. Lundgren. 2009. CD4+ T-cell responses to an oral inactivated cholera vaccine in young children in a cholera endemic country and the enhancing effect of zinc supplementation. Vaccine 28:422-9.
  3. Ahmed, T., A. M. Svennerholm, A. A. Tarique, G. N. Sultana, and F. Qadri. 2009. Enhanced immunogenicity of an oral inactivated cholera vaccine in infants in Bangladesh obtained by zinc supplementation and by temporary withholding breast-feeding. Vaccine 27:1433-1439.
  4. Attridge, S. R., and J. Holmgren. 2009. Vibrio cholerae O139 capsular polysaccharide confers complement resistance in the absence or presence of antibody yet presents a productive target for cell lysis: implications for detection of bactericidal antibodies. Microb Pathog 47:314-20.
  5. Attridge, S. R., G. Wallerstrom, B. L. Li, R. Morona, and J. Holmgren. 2009. Differential immunogenicity of Vibrio cholerae O139 variants expressing different combinations of naturally occurring and atypical forms of the serogroup polysaccharide. Vaccine 27:1055-1061.
  6. Bäckström M, Thomsson KA, Karlsson H, Hansson GC. Sensitive liquid chromatography-electrospray mass spectrometry allows for the analysis of the O-glycosylation of immunoprecipitated proteins from cells or tissues: application to MUC1 glycosylation in cancer. J Proteome Res. 2009 Feb;8(2):538-45.
  7. Blomqvist, M., Rhost, S. Teneberg, S. Löfbom, L. Osterbye, T. Brigl, M. Månsson, J.-E. and S. Cardell. 2009. Multiple tissue specific isoforms of sulfatide activate CD1d-restricted type II NKT cells. Eur J Immunol 39:1726-1735.
  8. Berglund, M., J. A. Thomas, M. F. Fredin, S. Melgar, E. H. Hornquist, and O. H. Hultgren. 2009. Gender dependent importance of IRAK-1 in dextran sulfate sodium induced colitis. Cell Immunol 259:27-32.
  9. Bhuiyan, T. R., S. B. Lundin, A. I. Khan, A. Lundgren, J. B. Harris, S. B. Calderwood, and F. Qadri. 2009. Cholera caused by Vibrio cholerae O1 induces T-cell responses in the circulation. Infect Immun 77:1888-1893.
  10. Bhuiyan, T. R., F. Qadri, A. Saha, and A. M. Svennerholm. 2009. Infection by helicobacter pylori in Bangladeshi children from birth to two years: relation to blood group, nutritional status, and seasonality. Pediatr Infect Dis J 28:79-85.
  11. Cheng, C., I. Bettahi, M. I. Cruz-Fisher, S. Pal, P. Jain, Z. Jia, J. Holmgren, A. M. Harandi, and L. M. de la Maza. 2009. Induction of protective immunity by vaccination against Chlamydia trachomatis using the major outer membrane protein adjuvanted with CpG oligodeoxynucleotide coupled to the nontoxic B subunit of cholera toxin. Vaccine 27:6239-46.
  12. Cucak, H., U. Yrlid, B. Reizis, U. Kalinke, and B. Johansson-Lindbom. 2009. Type I interferon signaling in dendritic cells stimulates the development of lymph-node-resident T follicular helper cells. Immunity 31:491-501.
  13. Cuburu, N., M. N. Kweon, C. Hervouet, H. R. Cha, Y. Y. Pang, J. Holmgren, K. Stadler, J. T. Schiller, F. Anjuere, and C. Czerkinsky. 2009. Sublingual immunization with nonreplicating antigens induces antibody-forming cells and cytotoxic T cells in the female genital tract mucosa and protects against genital papillomavirus infection. J Immunol 183:7851-7859.
  14. Cunningham, K. A., A. J. Carey, N. Lycke, P. Timms, and K. W. Beagley. 2009. CTA1-DD is an effective adjuvant for targeting anti-chlamydial immunity to the murine genital mucosa. J Reprod Immunol. 81:34-38.
  15. Del Campo, J., M. Lindqvist, M. Cuello, M. Backstrom, O. Cabrerra, J. Persson, O. Perez, and A. M. Harandi. 2009. Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice. Vaccine. (In press).
  16. Domino SE, Hurd EA, Thomsson KA, Karnak DM, Holmén Larsson JM, Thomsson E, Bäckström M, Hansson GC. Cervical mucins carry alpha(1,2)fucosylated glycans that partly protect from experimental vaginal candidiasis. Glycoconj J. 2009 Dec;26(9):1125-34.
  17. Fahlen-Yrlid, L., T. Gustafsson, J. Westlund, A. Holmberg, A. Strombeck, M. Blomquist, G. G. MacPherson, J. Holmgren, and U. Yrlid. 2009. CD11c (high )dendritic cells are essential for activation of CD4+ T cells and generation of specific antibodies following mucosal immunization. J Immunol 183:5032-41.
  18. Granio, O., K. A. Excoffon, P. Henning, P. Melin, C. Norez, G. Gonzalez, P. Karp, M. Magnusson, N. Habib, L. Lindholm, F. Becq, P. Boulanger, J. Zabner, and S. S. Hong. 2009. Adenovirus 5-fiber 35 chimeric vector mediates efficient apical correction of the CFTR defect in cystic fibrosis primary airway epithelia. Hum Gene Ther.
  19. Granio, O., M. Porcherot, S. Corjon, K. Kitidee, P. Henning, A. Eljaafari, A. Cimarelli, L. Lindholm, P. Miossec, P. Boulanger, and S. S. Hong. 2009. Improved adenovirus type 5 vector-mediated transduction of resistant cells by piggybacking on coxsackie B-adenovirus receptor-
    pseudotyped baculovirus. J Virol 83: 6048-6066.
  20. Grindebacke, H., H. Stenstad, M. Quiding-Jarbrink, J. Waldenstrom, I. Adlerberth, A. E. Wold, and A. Rudin. 2009. Dynamic development of homing receptor expression and memory cell differentiation of infant CD4+CD25(high) regulatory T cells. J Immunol 183:4360-4370.
  21. Hasselberg , A,. L. Ekman,  L.  Fahlén-Yrlid , K. Schön  and N. Lycke. 2009. ADP-ribosylation controls the outcome of tolerance or enhanced priming following mucosal immunization J. Immunol (In press). Hasselberg, A., K. Schon, A. Tarkowski, and N. Lycke. 2009. Role of CTA1R7K-COL-DD as a novel therapeutic mucosal tolerance-inducing vector for treatment of collagen-induced arthritis. Arthritis Rheum 60:1672-1682.  
  22. Hernroth, B. Å. Lothigius and I. Bölin 2009. Factors influencing survival of enterotoxigenic Escherichia coli, Salmonella enterica (serovar Typhimurium) and Vibrio parahaemolyticus in marine environments. FEMS Microbiol Ecol. [Epub ahead of print]
  23. Jansson, L., J. Tobias, C. Jarefjall, M. Lebens, A. M. Svennerholm, and S. Teneberg. 2009. Sulfatide recognition by colonization factor antigen CS6 from enterotoxigenic Escherichia coli. PLoS ONE 4:e4487.
  24. Janzon, A., A. Sjoling, A. Lothigius, D. Ahmed, F. Qadri, and A. M. Svennerholm. 2009. Failure to detect Helicobacter pylori DNA in drinking and environmental water in Dhaka, Bangladesh, using highly sensitive real-time PCR assays. Appl Environ Microbiol 75:3039-3044.
  25. Janzon, A., T. Bhuiyan, A. Lundgren, F. Qadri, A. M. Svennerholm, and A. Sjoling. 2009. Presence of high numbers of transcriptionally active Helicobacter pylori in vomitus from Bangladeshi patients suffering from acute gastroenteritis. Helicobacter 14:237-47.
  26. Johansson ME, Thomsson KA, Hansson GC. Proteomic analyses of the two mucus layers of the colon barrier reveal that their main component, the Muc2 mucin, is strongly bound to the Fcgbp protein. J Proteome Res. 2009 Jul;8(7):3549-57.
  27. Johansson DG, Wallin G, Sandberg A, Macao B, Aqvist J, Härd T. Protein autoproteolysis: conformational strain linked to the rate of peptide cleavage by  the pH dependence of the N --> O acyl shift reaction. J Am Chem Soc. 2009 Jul 15;131(27):9475-7.
  28. Kanungo, S., A. Paisley, A. L. Lopez, M. Bhattacharya, B. Manna, D. R. Kim, S. H. Han, S. Attridge, R. Carbis, R. Rao, J. Holmgren, J. D. Clemens, and D. Sur. 2009. Immune responses following one and two doses of the reformulated, bivalent, killed, whole-cell, oral cholera vaccine among adults and children in Kolkata, India: a randomized, placebo-controlled trial. Vaccine 27:6887-93.
  29. Kindlund, B., A. Sjoling, M. Hansson, A. Edebo, L. E. Hansson, H. Sjovall, A. M. Svennerholm, and B. S. Lundin. 2009. FOXP3-expressing CD4(+) T-cell numbers increase in areas of duodenal gastric metaplasia and are associated to CD4(+) T-cell aggregates in the duodenum of Helicobacter pylori-infected duodenal ulcer patients. Helicobacter 14:192-201.
  30. Larsson JM, Karlsson H, Sjövall H, Hansson GC. A complex, but uniform O-glycosylation of the human MUC2 mucin from colonic biopsies analyzed by nanoLC/MSn. Glycobiology. 2009 Jul;19(7):756-66.
  31. Lindén SK, Sheng YH, Every AL, Miles KM, Skoog EC, Florin TH, Sutton P, McGuckin MA. MUC1 limits Helicobacter pylori infection both by steric hindrance and by acting as a releasable decoy. PLoS Pathog. 2009 Oct;5(10):e1000617.
  32. Lindgren, Å., A. Sjöling and B.S. Lundin. 2009. IFN-γ secretion is induced in IL-12 stimulated human NK cells by recognition of Helicobacter pylori or TLR2/6, TLR1/2 and TLR5 ligands. Inn Immunity. (In press).
  33. Lindqvist, M., N. Navabi, M. Jansson, E. Samuelson, A. Sjoling, C. Orndal, and A. M. Harandi. 2009. Local cytokine and inflammatory responses to candidate vaginal adjuvants in mice. Vaccine 28:270-278.
  34. Lindqvist, M., J. Persson, K. Thorn, and A. M. Harandi. 2009. The mucosal adjuvant effect of alpha-galactosylceramide for induction of protective immunity to sexually transmitted viral infection. J Immunol 182:6435-6443.
  35. Lothigius, A., A. Sjoling, A. M. Svennerholm, and I. Bolin. 2009. Survival and gene expression of enterotoxigenic Escherichia coli during long-term incubation in sea water and freshwater. J Appl Microbiol.
  36. Lundgren, A., J. Kaim, and M. Jertborn. 2009. Parallel analysis of mucosally derived B- and T-cell responses to an oral typhoid vaccine using simplified methods. Vaccine 27: 4529-4536.
  37. Lindgren, Å.,C.H., Yun,A. Lundgren, A. Sjöling, L. Öhman, A-M. Svennerholm, J. Holmgren and B.S. Lundin. 2009. CD8- NK cells are greatly enriched in the human gastrointestinal tract and secrete IFN-γ in response to bacterial antigens. J Innate Immun In press.
  38. Lundqvist, A., J. Kubler-Kielb, S. Teneberg, K. Ahlman, and T. Lagergard. 2009. Immunogenic and adjuvant properties of Haemophilus ducreyi lipooligosaccharides. Microbes Infect 11:352-360.
  39. Milling, S. W., C. D. Jenkins, U. Yrlid, V. Cerovic, H. Edmond, V. McDonald, M. Nassar, and G. Macpherson. 2009. Steady-state migrating intestinal dendritic cells induce potent inflammatory responses in naive CD4+ T cells. Mucosal Immunol 2:156-165.
  40. Myhre, S., P. Henning, M. Friedman, S. Stahl, L. Lindholm, and M. K. Magnusson. 2009. Re-targeted adenovirus vectors with dual specificity; binding specificities conferred by two different Affibody molecules in the fiber. Gene Ther 16:252-261.
  41. Nicklasson M., Klena J., Rodas C., Torres O., Bourgeouis AL., Svennerholm A-M., Sjöling Å. 2009.Genetic Relationship of Enterotoxigenic Escherichia coli Strains Expressing Heat-Stable Enterotoxin and Coli Surface Antigen 6 in Guatemala and Mexico Emerging Infect  Dis. (In press).
  42. Nygren, E., B. L. Li, J. Holmgren, and S. R. Attridge. 2009. Establishment of an adult mouse model for direct evaluation of the efficacy of vaccines against Vibrio cholerae. Infect Immun 77:3475-3484.
  43. Persson J, Bäckström M, Johansson H, Jirström K, Hansson GC, Ohlin M. Molecular evolution of specific human antibody against MUC1 mucin results in improved recognition of the antigen on tumor cells. Tumour Biol.2009;30(4):221-31. Epub 2009 Sep 21.  
  44. Pålhagen S, Qi H, Mårtensson B, Wålinder J, Granérus AK, Svenningsson P. Monoamines, BDNF, IL-6 and corticosterone in CSF in patients with Parkinson's disease and major depression. J Neurol. 2009 Oct 21.
  45. Quiding-Jarbrink, M., P. Sundstrom, A. Lundgren, M. Hansson, M. Backstrom, C. Johansson, K. Enarsson, M. Hermansson, E. Johnsson, and A. M. Svennerholm. 2009. Decreased IgA antibody production in the stomach of gastric adenocarcinoma patients. Clin Immunol. 131:463-471.
  46. Rodas, C., V. Iniguez, F. Qadri, G. Wiklund, A. M. Svennerholm, and A. Sjoling. 2009. Development of multiplex PCR assays for detection of enterotoxigenic Escherichia coli colonization factors and toxins. J Clin Microbiol 47:1218-1220.
  47. Rosenblad MA, Larsen N, Samuelsson T, Zwieb C. Kinship in the SRP RNA family. RNA Biol. 2009 Nov-Dec;6(5):508-16.
  48. Rydstrom, A., and M. J. Wick. 2009. Monocyte and neutrophil recruitment during oral Salmonella infection is driven by MyD88-derived chemokines. Eur J Immunol 39:3019-3030.
  49. Shaheen, H. I., I. A. Abdel Messih, J. D. Klena, A. Mansour, Z. El-Wakkeel, T. F. Wierzba, J. W. Sanders, S. B. Khalil, D. M. Rockabrand, M. R. Monteville, P. J. Rozmajzl, A. M. Svennerholm, and R. W. Frenck. 2009. Phenotypic and genotypic analysis of enterotoxigenic Escherichia coli in samples obtained from Egyptian children presenting to referral hospitals. J Clin Microbiol 47:189-197.
  50. Shamsuzzaman, S., T. Ahmed, K. Mannoor, Y. A. Begum, P. K. Bardhan, R. B. Sack, D. A. Sack, A. M. Svennerholm, J. Holmgren, and F. Qadri. 2009. Robust gut associated vaccine-specific antibody-secreting cell responses are detected at the mucosal surface of Bangladeshi subjects after immunization with an oral killed bivalent V. cholerae O1/O139 whole cell cholera vaccine: Comparison with other mucosal and systemic responses. Vaccine 27:1386-1392.
  51. Sihlbom C, van Dijk Härd I, Lidell ME, Noll T, Hansson GC, Bäckström M. Localization of O-glycans in MUC1 glycoproteins using electron-capture dissociation fragmentation mass spectrometry. Glycobiology. 2009 Apr;19(4):375-81.
  52. Sundquist, M., and M. J. Wick. 2009. Salmonella induces death of CD8alpha(+) dendritic cells but not CD11c(int)CD11b(+) inflammatory cells in vivo via MyD88 and TNFR1. J  Leukoc Biol 85: 225-234.
  53. Sur, D., A. L. Lopez, S. Kanungo, A. Paisley, B. Manna, M. Ali, S. K. Niyogi, J. K. Park, B. Sarkar, M. K. Puri, D. R. Kim, J. L. Deen, J. Holmgren, R. Carbis, R. Rao, T. V. Nguyen, A. Donner, N. K. Ganguly, G. B. Nair, S. K. Bhattacharya, and J. D. Clemens. 2009. Efficacy and safety of a modified killed-whole-cell oral cholera vaccine in India: an interim analysis of a cluster-randomised, double-blind, placebo-controlled trial. Lancet 374:1694-702.
  54. Svensson, H., M. Hansson, J. Kilhamn, S. Backert, and M. Quiding-Jarbrink. 2009. Selective up-regulation of endothelial E-selectin in response to H. pylori-induced gastritis. Infect Immun 77:3109-3116.
  55. Tam, M. and Wick, M.J. 2009. MyD88 and IFN-a/b are differentially required for dendritic cell maturation but dispensable for development of protective memory against Listeria. Immunol 128: 429-439.
  56. Uil, T. G., J. de Vrij, J. Vellinga, M. J. Rabelink, S. J. Cramer, O. Y. Chan, M. Pugnali, M. Magnusson, L. Lindholm, P. Boulanger, and R. C. Hoeben. 2009. A lentiviral vector-based adenovirus fiber-pseudotyping approach for expedited functional assessment of candidate retargeted fibers. J Gene Med 11:990-1004.
  57. Valeru, S. P., P. K. Rompikuntal, T. Ishikawa, K. Vaitkevicius, A. Sjoling, N. Dolganov, J. Zhu, G. Schoolnik, and S. N. Wai. 2009. Role of melanin pigment in expression of Vibrio cholerae virulence factors. Infect Immun 77:935-942.
  58. Wada Y, Dell A, Haslam SM, Tissot B, Canis K, Azadi P, Bäckström M, Costello CE, Hansson GC, Hiki Y, Ishihara M, Ito H, Kakehi K, Karlsson N, Kato K, Kawasaki N, Khoo KH, Kobayashi K, Kolarich D, Kondo A, Lebrilla C, Nakano M, Narimatsu H,  Novak J, Novotny MV, Ohno E, Packer NH, Renfrow MB, Tajiri M, Thomsson KA, Yagi H, Yu SY, Taniguchi N. Comparison of Methods for Profiling O-glycosylation: HUPO Human Disease Glycomics/Proteome Initiative Multi-Institutional Study of IgA1. Mol Cell Proteomics. 2010 Jan 4.
  59. Wising, C., M. Magnusson, K. Ahlman, L. Lindholm, and T. Lagergård. 2009. Toxic activity of the CdtB component of Haemophilus ducreyi cytolethail distending toxin expressed from Adenovirus5vector. APMIS.  In press.  


  60. Bhattacharya, S., R. Black, L. Bourgeois, J. Clemens, A. Cravioto, J. L. Deen, G. Dougan, R. Glass, R. F. Grais, M. Greco, I. Gust, J. Holmgren, S. Kariuki, P. H. Lambert, M. A. Liu, I. Longini, G. B. Nair, R. Norrby, G. J. Nossal, P. Ogra, P. Sansonetti, L. von Seidlein, F. Songane, A. M. Svennerholm, D. Steele, and R. Walker. 2009. Public health. The cholera crisis in Africa. Science 324:885.
  61. Bland, P.W., A.M., Williams, R., Stepankova, H. Tlaskalova-Hogenova, and C.S.J. Probert, 2009. Early antigen experience shapes the mucosal T cell repertoire.  Old Herborn Univ Sem Mon, 22, 67-73.
  62. Clemens, J., and J. Holmgren. 2009. Urgent need of cholera vaccines in public health-control programs. Future Microbiol 4:381-385.
  63. Czerkinsky, C., and J. Holmgren. 2009. Enteric vaccines for the developing world: a challenge for mucosal immunology. Mucosal Immunol 2:284-287.
  64. Dávila López M, Rosenblad MA, Samuelsson T. Conserved and variable domains of RNase MRP RNA. RNA Biol. 2009 Jul;6(3):208-20. Epub 2009 Jul 30. Review.
  65. Harandi, A. M., G. Davies, and O. F. Olesen. 2009. Vaccine adjuvants: scientific challenges and strategic initiatives. Expert Rev Vaccines 8:
  66. Karlsson H, Larsson JM, Thomsson KA, Härd I, Bäckström M, Hansson GC. High-throughput and high-sensitivity nano-LC/MS and MS/MS for O-glycan profiling. Methods Mol Biol. 2009;534:117-31. Review.
  67. Milling, S.W.F., U. Yrlid, V. Cerovic and G.G. MacPherson . 2009. Subsets of migrating intestinal dendritic cells. Immunological Reviews.
    (In Press). 
  68. Quiding-Jarbrink, M., M. Bove, and G. Dahlen. 2009. Infections of the esophagus and the stomach. Periodontol 2000, 49:166-178.
  69. Sun J.B., C. Czerkinsky, and J. Holmgren. 2009. Mucosally induced immunological tolerance: Strong potentiation by use of antigens coupled to cholera toxin B subunit. Scand J Immunol. (In press).

Book chapters


  1. Harandi, A.M., and O.F. Olesen. 2009. Vaccine Adjuvants: Priorities and Challenges. Book title: “Immunologic Adjuvant Research” ISBN: 978-1-60692-399-3. Nova Science Publishers, pp: 97-104.
  2. Svennerholm, A.M., and G. Glenn. 2009. Vaccines against enterotoxigenic Escherichia coli (ETEC). In New Generation Vaccines. 4th ed. Eds Levine MM et al. pp.
  3. Lucy C. Freitag, John D. Clements, Dubravka Grdic Eliasson, and Nils Lycke. Use of genetically or chemically detoxified mutants of Cholera and Escherichia coli heat-labile enterotoxins as mucosal adjuvants. In New Generation Vaccines. 4th ed. Eds Levine MM et al (in press)  pp 273-283.